CASK Rabbit Polyclonal Antibody

CAT#: TA302217

Rabbit Polyclonal Antibody against CASK (C-term)

Product Images

Western blot analysis of CASK (arrow) using Cask Antibody (C-term) (Cat.#TA302217). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CASK gene (Lane 2) (Origene Technologies).

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  Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 1:1000, IHC: 1:50~100
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: This CASK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 564-596 amino acids from the C-terminal region of human CASK.
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 105064 Da
• Gene Name: calcium/calmodulin dependent serine protein kinase
• Database Link:
  NP_003679
  Entrez Gene 12361 MouseEntrez Gene 29647 RatEntrez Gene 8573 Human
  UniProt ID O14936
• Background: Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The calcium/calmodulin-dependent kinase (CAMK) group consists of 75 kinases regulated by Ca2+/CaM and close relative family (CAMK, CAMKL, DAPK, MAPKAPK).
• Synonyms: CAGH39; CAMGUK; CMG; FGS4; LIN2; MICPCH; MRXSNA; TNRC8

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Tight junction