CD14 Rabbit Polyclonal Antibody

CAT#: TA302272

Rabbit Polyclonal Antibody against CD14 (C-term)

Product Images

Western blot analysis of CD14 (arrow) using rabbit polyclonal CD14 Antibody (C-term) (Cat.#TA302272). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CD14 gene (Lane 2) (Origene Technologies).

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  CD14 Antibody (C-term) (Cat.#TA302272) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the CD14 antibody detected the CD14 protein (arrow).

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  Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CD14 antibody (C-term) (Cat.#TA302272), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

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  CD14 Antibody (C-term) (Cat. #TA302272) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution: WB: 1:1000, IHC: 1:10~50, FC: 1:10~50
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: This CD14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 292-322 amino acids from the C-terminal region of human CD14.
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 40076 Da
• Gene Name: CD14 molecule
• Database Link:
  NP_000582
  Entrez Gene 929 Human
  UniProt ID P08571
• Background: CD14 is a surface protein preferentially expressed on monocytes/macrophages. It binds lipopolysaccharide binding protein and recently has been shown to bind apoptotic cells.
• Synonyms: CD14 antigen; CD14 molecule; monocyte differentiation antigen CD14

Reference Data

• Protein Families: Adult stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Hematopoietic cell lineage, MAPK signaling pathway, Pathogenic Escherichia coli infection, Regulation of actin cytoskeleton, Toll-like receptor signaling pathway