HP1 alpha (CBX5) Goat Polyclonal Antibody

CAT#: TA302445

Goat Polyclonal Antibody against CBX5 / HP1-Alpha

Product Images

TA302445 (0.03ug/ml) staining of Hela lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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  Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml). ugG control: Unimmunizedugoat ugG (black line) followed by Alexa Fluor 488 secondary antibody (4ug/ml).

Specifications

Product Data

• Applications: FC, WB
• Recommended Dilution: ELISA: 1:128,000. WB: 0.03-0.1µg/ml.
• Reactivities: Human
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Peptide with sequence C-NKRKSNFSNSADDIK, from the internal region of the protein sequence according to NP_036249.1.
• Formulation: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: lot specific
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 25848 Da
• Gene Name: chromobox 5
• Database Link:
  NP_036249
  Entrez Gene 12419 MouseEntrez Gene 477593 DogEntrez Gene 23468 Human
  UniProt ID P45973
• Background: This gene encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family. The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The encoded product is involved in the formation of functional kinetochore through interaction with essential kinetochore proteins. The gene has a pseudogene located on chromosome 3. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq]
• Synonyms: HEL25; HP1; HP1A