ITCH Goat Polyclonal Antibody

CAT#: TA302519

Goat Polyclonal Antibody against ITCH / AIF4

Product Images

TA302519 (0.03ug/ml) staining of Human Brain (Frontal Cortex) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: ELISA: 1:32,000. WB: 0.03-0.1µg/ml.
• Reactivities: Human
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Peptide with sequence C-EIKSHDLKPNGGN, from the internal region of the protein sequence according to NP_113671.3.
• Formulation: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: lot specific
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 102310 Da
• Gene Name: itchy E3 ubiquitin protein ligase
• Database Link:
  NP_113671
  Entrez Gene 16396 MouseEntrez Gene 311567 RatEntrez Gene 477199 DogEntrez Gene 83737 Human
  UniProt ID Q96J02
• Background: Atrophin-1 contains a polyglutamine repeat, expansion of which is responsible for dentatorubral and pallidoluysian atrophy. The protein encoded by this gene interacts with atrophin-1. This encoded protein is a closely related member of the NEDD4-like protein family. This family of proteins are E3 ubiquitin-ligase molecules and regulate key trafficking decisions, including targeting of proteins to proteosomes or lysosomes. This encoded protein contains four tandem WW domains and a HECT (homologous to the E6-associated protein carboxyl terminus) domain. It can act as a transcriptional corepressor of p45/NFE2 and may participate in the regulation of immune responses by modifying Notch-mediated signaling. It is highly similar to the mouse Itch protein, which has been implicated in the regulation and differentiation of erythroid and lymphoid cells. [provided by RefSeq]
• Synonyms: ADMFD; AIF4; AIP4; dJ468O1.1; NAPP1

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Endocytosis, Ubiquitin mediated proteolysis