XLF (NHEJ1) Goat Polyclonal Antibody

CAT#: TA302637

Goat Polyclonal Antibody against XRCC4-like factor / NHEJ1

Product Images

TA302637 optimised QC. Primary incubation 1 hour at room temperature. Image A: HeLa nuclear cell lysate at primary Ab concentration 0.01µg/ml, Images B, C, D: A431, Jurkat, K562 nuclear cell lysate at primary Ab concentration 0.03µg/ml, Image E: HepG2 nuclear cell lysate at primary Ab concentration 0.1µg/ml. (Loaded 35µg protein in RIPA buffer, per lane). Detected by chemiluminescence.

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  TA302637 optimised QC. Primary incubation 1 hour at room temperature. Image A: Human Skeletal muscle lysate at primary Ab concentration 0.03ug/ml. (Loaded 35µg protein in RIPA buffer, per lane). Detected by chemiluminescence.

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  TA302637 (3.8µg/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

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  TA302637 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing strong nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

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  TA302637 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

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  TA302637 Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

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Specifications

Product Data

• Applications: WB
• Recommended Dilution: ELISA: 1:64,000. WB: 0.1-0.3µg/ml.
• Reactivities: Human (Expected from sequence similarity: Dog)
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Peptide with sequence C-QRPQLSKVKRKKPR, from the C Terminus of the protein sequence according to NP_079058.1.
• Formulation: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: lot specific
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 36962 Da
• Gene Name: non-homologous end joining factor 1
• Database Link:
  NP_079058
  Entrez Gene 610570 DogEntrez Gene 79840 Human
  UniProt ID Q9H9Q4
• Background: Double-strand breaks in DNA result from genotoxic stresses and are among the most damaging of DNA lesions. This gene encodes a DNA repair factor essential for the nonhomologous end-joining pathway, which preferentially mediates repair of double-stranded breaks. Mutations in this gene cause different kinds of severe combined immunodeficiency disorders. [provided by RefSeq]
• Synonyms: XLF

Reference Data

• Protein Pathways: Non-homologous end-joining