NCF1 Goat Polyclonal Antibody

CAT#: TA302677

Goat Polyclonal Antibody against NCF1 / p47phox

Product Images

TA302677 staining (0.2ug/ml) of U937 lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

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  TA302677 (1ug/ml) staining of Mouse Thymus (A) and Pig Spleen (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

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  TA302677 (5ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA:1:20000. WB:0.5-1.5µg/ml.IHC:5µg/ml
• Reactivities: Human
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Peptide with sequence C-SESTKRKLASAV, from the C Terminus of the protein sequence according to NP_000256.3.
• Formulation: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 44.5 kDa
• Gene Name: neutrophil cytosolic factor 1
• Database Link:
  NP_000256
  Entrez Gene 17969 MouseEntrez Gene 114553 RatEntrez Gene 653361 Human
  UniProt ID P14598
• Background: The protein encoded by this gene is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in this gene have been associated with chronic granulomatous disease. [provided by RefSeq]
• Synonyms: NCF1A; NOXO2; p47phox; SH3PXD1A

Reference Data

• Protein Pathways: Chemokine signaling pathway, Fc gamma R-mediated phagocytosis, Leukocyte transendothelial migration, Pathogenic Escherichia coli infection, Regulation of actin cytoskeleton