RANBP1 Goat Polyclonal Antibody

CAT#: TA302686

Goat Polyclonal Antibody against RANBP1

Product Images

TA302686 (0.05ug/ml) staining of HELA lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.chemiluminescence.

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  TA302686 (3.8ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 0.05-0.1ug/ml; IHC: 3-5ug/ml; ELISA: 1:16,000
• Reactivities: Human
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Peptide with sequence C-LSVKEETKEDAEEKQ, from the C Terminus of the protein sequence according to NP_002873.
• Formulation: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: lot specific
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 23.1 kDa
• Gene Name: RAN binding protein 1
• Database Link:
  NP_002873
  Entrez Gene 5902 Human
  UniProt ID P43487
• Background: Ran/TC4-binding protein, RanBP1, interacts specifically with GTP-charged RAN. RANBP1 encodes a 23-kD protein that binds to RAN complexed with GTP but not GDP. RANBP1 does not activate GTPase activity of RAN but does markedly increase GTP hydrolysis by the RanGTPase-activating protein (RanGAP1). The RANBP1 cDNA encodes a 201-amino acid protein that is 92% similar to its mouse homolog. In both mammalian cells and in yeast, RANBP1 acts as a negative regulator of RCC1 by inhibiting RCC1-stimulated guanine nucleotide release from RAN.
• Synonyms: HTF9A