KAP1 (TRIM28) Goat Polyclonal Antibody

CAT#: TA302849

Goat Polyclonal Antibody against TRIM28

Product Images

TA302849 staining (0.5ug/ml) of HepG2 lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

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  TA302849 (2ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.

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  Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showiug nuclear stainiug. The nuclear stain is DAPI (blue)

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  Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showiug nuclear stainiug. The nuclear stain is DAPI (blue)

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  Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

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Specifications

Product Data

• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB: 0.2-1µg/ml.
• Reactivities: Human, Mouse
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Peptide with sequence C-SSQELSGGPGDGP, from the C Terminus of the protein sequence according to NP_005753.
• Formulation: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: lot specific
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: tripartite motif containing 28
• Database Link:
  NP_005753
  Entrez Gene 21849 MouseEntrez Gene 10155 Human
  UniProt ID Q13263
• Background: The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. [provided by RefSeq]
• Synonyms: KAP1; PPP1R157; RNF96; TF1B; TIF1B

Reference Data

• Protein Families: Protein Kinase, Stem cell - Pluripotency, Transcription Factors