CHFR Goat Polyclonal Antibody

CAT#: TA302851

Goat Polyclonal Antibody against CHFR

Product Images

TA302851 (2.5ug/ml) staining of paraffin embedded human brain (cerebellum). Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Specifications

Product Data

• Applications: IHC
• Recommended Dilution: ELISA: 1:64,000. WB: 2.5µg/ml.
• Reactivities: Human
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Peptide with sequence KFNHICEQTRFKN, from the C Terminus of the protein sequence according to NP_060693.
• Formulation: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: lot specific
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase
• Database Link:
  NP_060693
  Entrez Gene 231600 MouseEntrez Gene 288734 RatEntrez Gene 55743 Human
  UniProt ID Q96EP1
• Background: E3 ubiquitin-protein ligase required to transiently arrest cells in early prophase when they are exposed to microtubule poisons. Acts in early prophase before chromosome condensation, when the centrosome move apart from each other along the periphery of the nucleus. Probably promotes the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBC13-MMS2 (UBE2N-UBE2V2) heterodimer. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation, but are rather involved in signaling cellular stress. This suggests that it may be involved in signaling the presence of mitotic stress caused by microtubule poisons
• Synonyms: RNF116; RNF196

Reference Data

• Protein Families: Druggable Genome