GAPDH Goat Polyclonal Antibody

CAT#: TA302944

Goat polyclonal anti-GAPDH antibody(C-terminal), Loading control

Product Images

TA302944 staining (0.01ug/ml) of 293 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

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  TA302944 (0.1ug/ml) staining of HeLa lysate (control in left lane and after si-RNA-mediated GAPDH knock-down expresson in right lane) (35ug protein in RIPA buffer). Level of knock-down relative to Actin expression level was determined by RT-PCR. Primary incubation was 1 hour. Detected by chemiluminescence.

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  TA302944 (0.5ug/ml) staining of Mouse Liver (A) and Rat Brain (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

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  TA302944 (0.1ug/ml) staining of Human Liver (A) and Tonsil (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

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  TA302944 (0.1ug/ml) staining of lysates of cell line HEK293 (A) and HeLa (B) (35ug protein in RIPA buffer). Detected by chemiluminescence.

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  TA302944 (5ug/ml) staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

• TA302944 Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.

• TA302944 (0.1ug/ml) staining of PFA-fixed HeLa before (top) and after (bottom) si-RNA-mediated GAPDH knock-down expresson. Primary incubation 1h at ambient temp. Detection by DyLight 488. Nuclear DAPI stain (right).

• TA302944 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (b

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Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: ELISA:1:128000.WB:0.01-0.5µg/ml.IHC: 5µg/ml. IHC: 5µg/ml.
• Reactivities: Human, Mouse, Rat
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Peptide with sequence C-HQVVSSDFNSDT, from the C Terminus of the protein sequence according to NP_002037.2.
• Formulation: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: glyceraldehyde-3-phosphate dehydrogenase
• Database Link:
  NP_002037
  Entrez Gene 14433 MouseEntrez Gene 24383 RatEntrez Gene 403755 DogEntrez Gene 2597 Human
  UniProt ID P04406
• Background: The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. [provided by RefSeq]
• Synonyms: G3PD; GAPD; HEL-S-162eP
• Note: In paraffin embedded Human Tonsil, staining of cells in germinal centre, mantle zone, interfollicular area, epithelium and endothelium is observed

Reference Data

• Protein Families: ES Cell Differentiation/IPS
• Protein Pathways: Alzheimer's disease, Glycolysis / Gluconeogenesis, Metabolic pathways