AIRE Goat Polyclonal Antibody

CAT#: TA303113

Goat Polyclonal Antibody against AIRE (Internal Region)

Product Images

TA303113 staining (0.05ug/ml) of HEK293 cell lysates. Untransfected (Lane 3) and transfected with Human AIRE (lane 4). Data kindly provided by Prof. Pärt Peterson, University of Tartu, Estonia.

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  TA303113 (2.5ug/ml) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA: 1:32,000. WB: 0.5-1.0ug/ml. IHC: 2.5ug/ml.
• Reactivities: Human, Pig
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Peptide with sequence C-KDVDLSQPRKGRKP, from the internal region of the protein sequence according to NP_000374.1.
• Formulation: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: lot specific
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: autoimmune regulator
• Database Link:
  NP_000374
  Entrez Gene 294328 RatEntrez Gene 487796 DogEntrez Gene 326 Human
  UniProt ID O43918
• Background: This gene encodes a transcriptional regulator that forms nuclear bodies and interacts with the transcriptional coactivator CBP. At least three splice variant mRNAs products have been described including one which results in a premature stop codon and a transcript predicted to be a candidate for nuclear-mediated decay (NMD). Defects in this gene cause the rare autosomal-recessive systemic autoimmune disease termed autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED). [provided by RefSeq]
• Synonyms: AIRE1; APECED; APS1; APSI; PGA1
• Note: IHC: In paraffin embedded Human Thymus shows nuclear staining in select thymocytes. This product was confirmed in IHC on fetal Pig Thymus.

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Primary immunodeficiency, Ubiquitin mediated proteolysis