Gfap Goat Polyclonal Antibody

CAT#: TA303157

Goat Polyclonal Antibody against GFAP

Product Images

TA303157 (0.001ug/ml) staining of Human Brain (Cerebellum) (A), (0.3ug/ml) Mouse (B) and (0.01ug/ml) Rat (C) Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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  TA303157 (5ug/ml) as the reporter with [EB002015] as the capture rabbit antibody (5ug/ml). Please note this data was obtained using a previous batch.

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  TA303157 (2ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining. Please note this data was obtained using a previous batch.

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  TA303157 (0.01ug/ml) staining of PFA-perfused cryosection of Human Hypothalamus. Antigen retrieval with citrate buffer pH 6 at 80°C for 30min, HRP-staining with Ni-DAB after Biotin-SP-antigoat amplification. Data obtained by Prof. Erik Hrabovszky, Inst, Ex

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA: 1:64,000, WB: 0.3-1ug/ml, IHC: 2-4ug/ml, Assay: 5-10ug/ml
• Reactivities: Human, Mouse
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Peptide with sequence C-DGEVIKESKQEHKD, from the C Terminus of the protein sequence according to NP_002046.1.
• Formulation: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: lot specific
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: glial fibrillary acidic protein
• Database Link:
  NP_034407
  Entrez Gene 2670 HumanEntrez Gene 24387 RatEntrez Gene 480495 DogEntrez Gene 14580 Mouse
  UniProt ID P03995
• Background: This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq]
• Synonyms: FLJ45472