TIA1 Goat Polyclonal Antibody

CAT#: TA303222

Goat Polyclonal Antibody against TIA1

Product Images

TA303222 (0.1ug/ml) staining of Jurkat cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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  TA303222 (3.75ug/ml) staining of paraffin embedded Human Thyroid. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

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  TA303222 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and some cytoplasmic staining. The nuclear

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: ELISA: 1:128,000. WB: 0.1-0.3µg/ml.IHC:3.75µg/ml.IF:5µg/ml.
• Reactivities: Human
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Peptide with sequence C-PKSTYESNTKQ, from the internal region of the protein sequence according to NP_071320.1; NP_071505.1.
• Formulation: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: lot specific
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: TIA1 cytotoxic granule-associated RNA binding protein
• Database Link:
  NP_071320
  Entrez Gene 21841 MouseEntrez Gene 312510 RatEntrez Gene 610625 DogEntrez Gene 7072 Human
  UniProt ID P31483
• Background: The product encoded by this gene is a member of a RNA-binding protein family and possesses nucleolytic activity against cytotoxic lymphocyte (CTL) target cells. It has been suggested that this protein may be involved in the induction of apoptosis as it preferentially recognizes poly(A) homopolymers and induces DNA fragmentation in CTL targets. The major granule-associated species is a 15-kDa protein that is thought to be derived from the carboxyl terminus of the 40-kDa product by proteolytic processing. Alternative splicing resulting in different isoforms of this gene product has been described in the literature. [provided by RefSeq]
• Synonyms: TIA-1; WDM

Reference Data

• Protein Families: Druggable Genome