ABCB5 Goat Polyclonal Antibody

CAT#: TA303312

Goat Polyclonal Antibody against ABCB5

Product Images

TA303312 (0.3ug/ml) staining of lysates of cell line A431 (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• 

  Flow cytometric analysis of paraformaldehyde fixed Heug2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml).

Specifications

Product Data

• Applications: FC, WB
• Recommended Dilution: ELISA: 1:32,000. WB: 0.3-1µg/ml.
• Reactivities: Human
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Peptide with sequence C-QTQHRNTSKKAQ, from the internal region of the protein sequence according to NP_848654.3.
• Formulation: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: ATP binding cassette subfamily B member 5
• Database Link:
  NP_848654
  Entrez Gene 340273 Human
  UniProt ID Q2M3G0
• Background: ABCB5 belongs to the ATP-binding cassette (ABC) transporter superfamily of integral membrane proteins. These proteins participate in ATP-dependent transmembrane transport of structurally diverse molecules ranging from small ions, sugars, and peptides to more complex organic molecules (Chen et al., 2005 [PubMed 15760339]). [supplied by OMIM]
• Synonyms: ABCB5alpha; ABCB5beta; EST422562
• Note: This antibody has been successfully used in Flow Cytometry for Hep3B cells by customer.

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: ABC transporters