ErbB 4 (ERBB4) Rabbit Monoclonal Antibody [Clone ID: EP2272AY]

CAT#: TA303505

Rabbit monoclonal antibody against Her4 / ErbB-4 Phospho (pY1258) (EP2272AY ) (phospho-specific)

Product Images

Western blot - ErbB 4 (phospho Y1258) antibody [EP2272AY]; All lanes : Anti-ErbB 4 (phospho Y1258) antibody [EP2272AY] at 1/2000 dilution.Lane 1 : SKBR3 cell lysates treated with EGF.Lane 2 : SKBR3 cell lysates untreated.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 147 kDa.Observed band size : 180 kDa.

Specifications

Product Data

• Clone Name: EP2272AY
• Applications: WB
• Recommended Dilution: WB: 1:1000 - 1:5000
• Reactivities: Human, Mouse, Rat
• Modifications: Phospho-specific
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A phospho specific peptide corresponding to residues surrounding tyrosine 1258 of human Her4/ErbB-4 was used as an immunogen. This antibody detects Her4/ErbB-4 phosphorylated on tyrosine 1258.
• Formulation: PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• Purification: Tissue culture supernatant
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 147 kDa
• Gene Name: erb-b2 receptor tyrosine kinase 4
• Database Link:
  NP_005226
  Entrez Gene 13869 MouseEntrez Gene 59323 RatEntrez Gene 2066 Human
  UniProt ID Q15303
• Background: This gene is a member of the Tyr protein kinase family and the epidermal growth factor receptor subfamily. It encodes a single-pass type I membrane protein with multiple cysteine rich domains, a transmembrane domain, a tyrosine kinase domain, a phosphotidylinositol-3 kinase binding site and a PDZ domain binding motif. The protein binds to and is activated by neuregulins and other factors and induces a variety of cellular responses including mitogenesis and differentiation. Multiple proteolytic events allow for the release of a cytoplasmic fragment and an extracellular fragment. Mutations in this gene have been associated with cancer. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized. [provided by RefSeq]
• Synonyms: ALS19; HER4; p180erbB4
• Note: Is unsuitable for Flow Cyt,ICC,IHC-P or IP.

Reference Data

• Protein Families: Druggable Genome, Protein Kinase, Transmembrane
• Protein Pathways: Calcium signaling pathway, Endocytosis, ErbB signaling pathway