c-Myc (MYC) Rabbit Monoclonal Antibody [Clone ID: E203]

CAT#: TA303607

Rabbit monoclonal antibody against Phospho-c-Myc (pT58/S62)(E203) (phospho-specific)

Product Images

Western blot - c-Myc (phospho T58 + S62) antibody [E203]; All lanes : Anti-c-Myc (phospho T58 + S62) antibody [E203] at 1/1000 dilution.Lane 1 : Untreated A431 cell lysate.Lane 2 : TPA treated A431 cell lysate.Predicted band size : 49 kDa.Observed band size : 57 kDa.

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  Immunofluorescence - c-Myc (phospho T58 + S62) antibody [E203]; Immunofluorescent staining of A431 cells

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  Flow Cytometry - Anti-c-Myc (phospho T58 + S62) antibody; Overlay histogram showing Jurkat cells stained with TA303607 (red line). The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H&L) at 1:2000. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control.

Specifications

Product Data

• Clone Name: E203
• Applications: FC, IF, WB
• Recommended Dilution: WB: 1:1,000; ICC: 1:50-100; IP: 1:100
• Reactivities: Human, Rat
• Modifications: Phospho-specific
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Thr58 and Ser62 of human C-Myc was used as immunogen. The antibody only detects c-Myc phosphorylated on Threonine 58 and Serine 62. Predicted to cross-react with most species, based on sequ
• Formulation: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 49 kDa (Detect a band at 57 kDa)
• Gene Name: v-myc avian myelocytomatosis viral oncogene homolog
• Database Link:
  NP_002458
  Entrez Gene 24577 RatEntrez Gene 4609 Human
  UniProt ID P01106
• Background: The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene. [provided by RefSeq]
• Synonyms: bHLHe39; c-Myc; MRTL; MYCC

Reference Data

• Protein Families: Druggable Genome, Embryonic stem cells, Induced pluripotent stem cells, Stem cell - Pluripotency, Stem cell relevant signaling - JAK/STAT signaling pathway, Stem cell relevant signaling - TGFb/BMP signaling pathway, Stem cell relevant signaling - Wnt Signaling pathway, Transcription Factors
• Protein Pathways: Acute myeloid leukemia, Bladder cancer, Cell cycle, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, ErbB signaling pathway, Jak-STAT signaling pathway, MAPK signaling pathway, Pathways in cancer, Small cell lung cancer, TGF-beta signaling pathway, Thyroid cancer, Wnt signaling pathway