AMPK alpha 1 (PRKAA1) Rabbit Monoclonal Antibody [Clone ID: Y365]

CAT#: TA303654

Rabbit monoclonal antibody against AMPK alpha-1(Y365)

Product Images

Western blot - AMPK alpha 1 antibody [Y365]; All lanes : Anti-AMPK alpha 1 antibody [Y365] at 1/5000 dilution.Lane 1 : MCF-7.Lane 2 : Hela.Predicted band size : 63 kDa.Observed band size : 63 kDa.

• 

  Immunohistochemistry (Paraffin-embedded sections) - AMPK alpha 1 antibody [Y365]; TA303654 at a 1:100 - 1:250 dilution staining AMPK alpha 1 in Human cervical carcinoma, using Immunohistochemistry, Paraffin Embedded Tissue.

• 

  Flow Cytometry - Anti-AMPK alpha 1 antibody; Overlay histogram showing HeLa cells stained with TA303654 (red line). The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1:500. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control.

Specifications

Product Data

• Clone Name: Y365
• Applications: FC, IHC, WB
• Recommended Dilution: FC: 1:100; WB: 1:1000 - 1:5000; IHC-P: 1:100 - 1:250; IP: 1:50
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide corresponding to residues near the C-term of human AMPK alpha-1 subunit was used as an immunogen
• Formulation: PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• Purification: IgG fraction
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 63 kDa
• Gene Name: protein kinase AMP-activated catalytic subunit alpha 1
• Database Link:
  NP_006242
  Entrez Gene 65248 RatEntrez Gene 105787 MouseEntrez Gene 5562 Human
  UniProt ID Q13131
• Background: The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq]
• Synonyms: AMPK; AMPKa1
• Note: Is unsuitable for ICC/IF.

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Adipocytokine signaling pathway, Hypertrophic cardiomyopathy (HCM), Insulin signaling pathway, mTOR signaling pathway, Regulation of autophagy