FLIP (CFLAR) Rabbit Polyclonal Antibody

CAT#: TA305962

Rabbit Polyclonal FLIP Antibody

Product Images

Western blot analysis of FLIP in (A) HeLa cell and (B) mouse liver tissue lysate with FLIP antibody at 1ug/ml.

• 

  Immunohistochemistry of FLIP in mouse liver tissue with FLIP antibody at 5ug/ml.

• 

  Immunofluorescence of FLIP in HeLa cells with FLIP antibody at 20ug/ml.

• 

  Immunocytochemistry of FLIP in HeLa cells with FLIP antibody at 5ug/ml.

• 

  Immunofluorescence of FLIP in mouse liver tissue with FLIP antibody at 20ug/ml.

• 

  Immunofluorescence of FLIP in mouse liver tissue with FLIP antibody at 20ug/ml.

Show all 6 images

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1 - 2 ug/mL, ICC: 5 ug/mL, IF: 20 ug/mL
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: FLIP antibody was raised against a peptide corresponding to 17 amino acids near the amino terminus of human FLIP. The sequence is identical in all FLIP splice variants. The immunogen is located within the first 50 amino acids of FLIP.
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1ug/ul
• Purification: Affinity chromatography purified via peptide column
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: CASP8 and FADD like apoptosis regulator
• Database Link:
  NP_001120655
  Entrez Gene 12633 MouseEntrez Gene 117279 RatEntrez Gene 8837 Human
  UniProt ID O15519
• Background: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD)- containing adapter molecules and members of the ICE/CED-3 protease family. Caspases-8 (FLICE) and -10 (FLICE2) are two pivotal members in the ICE/CED-3 protease family. FLICE-inhibitory proteins were identified in virus and human and designated v-FLIPs and FLIP, respectively. The human FLIP was also cloned by several labs independently and termed Casper , I-FLICE, FLAME-1, CASH and CLARP3-7. FLIP contains two death effector domains (DEDs) and a caspase-like domain. FLIP interacts with adapter protein FADD and caspase-8 and 10, and potently inhibits apoptosis induced by all known death receptors. Four splice variants of c-FLIPs have been identified and termed FLIPalpha, beta, gamma, and delta, respectively.
• Synonyms: c-FLIP; c-FLIPL; c-FLIPR; c-FLIPS; CASH; CASP8AP1; Casper; CLARP; FLAME; FLAME-1; FLAME1; FLIP

Reference Data

• Protein Families: Druggable Genome, Protease
• Protein Pathways: Apoptosis