FLIP (CFLAR) Rabbit Polyclonal Antibody

CAT#: TA305978

Rabbit Polyclonal FLIP Antibody

Product Images

Western blot analysis of FLIP in total cell lysates from HeLa (H), Jurkat (J), THP-1 (T), A431 (A), K562 (K) and NIH3T3 (N) cells with FLIP antibody at 1:1000 dilution.

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  Immunofluorescence of FLIP in HeLa cells with FLIP antibody at 10ug/ml.

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  Immunofluorescence of FLIP in Jurkat cells with FLIP antibody at 20ug/ml.

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  Immunocytochemistry of FLIP in Jurkat cells with FLIP antibody at 2ug/ml.

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  Immunocytochemistry staining of HeLa cells using FLIP antibody at 2ug/ml.

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Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: WB: 1:1000, ICC: 2 ug/mL, IF: 10 ug/mL
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: FLIP antibody was raised against a 19 amino acid peptide near the carboxy terminus of human FLIP. The immunogen is located within amino acids 180 - 230 of FLIP.
• Formulation: PBS containing 0.02% sodium azide.
• Purification: Affinity chromatography purified via peptide column
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: CASP8 and FADD like apoptosis regulator
• Database Link:
  NP_001120655
  Entrez Gene 8837 Human
  UniProt ID O15519
• Background: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD)-containing adapter molecules and members of the ICE/CED-3 protease family. Caspases-8 (FLICE) and -10 (FLICE2) are two pivotal members in the ICE/CED-3 protease family. FLICE-inhibitory proteins were identified in virus and human and designated v-FLIPs and c-FLIPs, respectively1,2. The human FLIPs were also cloned by several labs independently and termed Casper , I-FLICE, FLAME-1, CASH, CLARP and usurpin3-7. FLIP contains two death effector domains (DEDs) and a caspase-like domain. FLIP interacts with adapter protein FADD and caspase-8 and 10, and potently inhibits apoptosis induced by all known death receptors CD95, DR3, TRAIL-R and TNFR1. Four splice variants of c-FLIPs have been identified and termed FLIPalpha, beta, gamma, and delta, respectively (8).
• Synonyms: c-FLIP; c-FLIPL; c-FLIPR; c-FLIPS; CASH; CASP8AP1; Casper; CLARP; FLAME; FLAME-1; FLAME1; FLIP

Reference Data

• Protein Families: Druggable Genome, Protease
• Protein Pathways: Apoptosis