DNase II (DNASE2) Rabbit Polyclonal Antibody

CAT#: TA305979

Rabbit Polyclonal DNase II Antibody

Product Images

Western blot analysis of DNase II in human spleen tissue lysate in the absence (lane 1) or presence (lane 2) of blocking peptide with DNase antibody II at 1:500 dilution.

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  Immunohistochemistry of DNaseII in human spleen tissue with DNaseII antibody at 5 ug/ml.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 0.5 ug/mL, IHC: 5 ug/mL
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: DNase II antibody was raised against a peptide corresponding to amino acids 347 to 360 of human DNase II precursor (2-4) .
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1ug/ul
• Purification: Affinity chromatography purified via peptide column
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: deoxyribonuclease II, lysosomal
• Database Link:
  NP_001366
  Entrez Gene 1777 Human
  UniProt ID O00115
• Background: Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins including AIF and Acinus . DNase II causes both chromatin condensation and DNA fragmentation . The genes encoding human (2-4), porcine (4), and murine (5) DNase II have been cloned. The DNase II gene encodes a 40 kDa proenzyme. The mature enzyme consists of two non-identical subunits, the 32 kDa (a) and 12 kDa (b) chains, generated by proteolytic processing. Overexpression of DNase II induces chromatin condensation (3). DNase II is ubiquitously expressed in human tissues.
• Synonyms: DNASE2A; DNL; DNL2

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Lysosome