RIP2 (RIPK2) Rabbit Polyclonal Antibody

CAT#: TA305984

Rabbit Polyclonal RICK Antibody

Product Images

Western blot analysis of RICK in (A) HeLa, (B) Ramos and (C) EL4 cell lysate with RICK antibody at 1ug/ml.

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  Immunofluorescence of RICK in K562 cells with RICK antibody at 20ug/ml.

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  Immunofluorescence of RICK in K562 cells with RICK antibody at 20ug/ml.

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  Immunocytochemistry of RICK in K562 cells with RICK antibody at 2.5ug/ml.

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  Immunocytochemistry of RICK in A431 cells with RICK antibody at 10ug/ml.

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Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: WB: 1 ug/mL, ICC: 10 ug/mL
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: RICK antibody was raised against a peptide corresponding to 20 amino acids near the amino terminus of human RICK. The immunogen is located within the first 50 amino acids of RICK.
• Formulation: PBS containing 0.02% sodium azide.
• Purification: Affinity chromatography purified via peptide column
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: receptor interacting serine/threonine kinase 2
• Database Link:
  NP_003812
  Entrez Gene 192656 MouseEntrez Gene 362491 RatEntrez Gene 8767 Human
  UniProt ID O43353
• Background: Apoptosis is mediated by death domain (DD) and/or caspase recruitment domain (CARD) containing molecules and a caspase family of proteases. DD-containing serine/threonine kinase RIP regulates Fas-induced apoptosis. A novel CARD-containing serine/threonine kinase was recently identified and designated RICK/RIP2/CARDIAK for RIP-like interacting CLARP kinase, receptor interacting protein-2, and CARD-containing ICE associated kinase, respectively, (1-3). RICK contains an N-terminal kinase catalytic domain and a C-terminal CARD domain. Overexpression of RICK induced apoptosis and activation of NF-kappaB and JNK. RICK interacts with members of the TRAF family, CLARP and caspase-1. Thus, RICK represents a novel kinase that regulates TNF and Fas induced-apoptosis and that is involved in the generation of proinflammatory cytokine IL-1b. The messenger RNA of RICK is expressed in multiple human tissues.
• Synonyms: CARD3; CARDIAK; CCK; GIG30; RICK; RIP2

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Neurotrophin signaling pathway, NOD-like receptor signaling pathway