NCOA62 (SNW1) Rabbit Polyclonal Antibody

CAT#: TA306036

Rabbit Polyclonal SkiP Antibody

Product Images

Western blot analysis of SkiP in mouse skeletal muscle tissue lysate with SkiP antibody at (A) 0.5 and (B) 1 ug/mL.

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  Immunofluorescence of Ski in human kidney tissue with Ski antibody at 20 ug/mL.

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: WB: 0.5 - 1 ug/mL, ICC: 20 ug/mL, IF: 20 ug/mL
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: SkiP antibody was raised against a 16 amino acid peptide from near the carboxy terminus of human SkiP .
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1ug/ul
• Purification: Affinity chromatography purified via peptide column
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: SNW domain containing 1
• Database Link:
  NP_036377
  Entrez Gene 66354 MouseEntrez Gene 500695 RatEntrez Gene 22938 Human
  UniProt ID Q13573
• Background: TGF-beta and the bone morphogenic proteins (BMPs) are key signaling proteins that regulate numerous cellular processes such as embryonic development and tumorigenesis (1). Both signal through the Smad protein family and are negatively regulated by Ski and SnoN, two related proto-oncoproteins (2,3). Ski functions by binding to the Smad proteins activated by TGF-beta and the (BMPs) and preventing their phosphorylation, inhibiting their ability to bind DNA and activate the transcription of downstream genes (4,5). SkiP was originally identified as a Ski-interacting protein (6) and was later found to augment the signals induced by TGF-beta but inhibit transcription induced by BMP-2 (7) in C2C12 cells, suggesting that SkiP is a key player in the signaling cascades inititated by TGF-beta and the BMP protein family.
• Synonyms: Bx42; NCOA-62; Prp45; PRPF45; SKIIP; SKIP

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Notch signaling pathway, Spliceosome