Nephrin (NPHS1) Rabbit Polyclonal Antibody

CAT#: TA306037

Rabbit Polyclonal Nephrin Antibody

Product Images

Western blot analysis of Nephrin in mouse kidney tissue lysate with Nephrin antibody at 1ug/ml in the (A) absence and (B) presence of blocking peptide.

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  Figure from citation: Western Blot of NPHS1 (Nephrin) protein level by using anti-NPHS1 antibody in cell lysates of quiescent mouse podocytes. View Citation

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  Immunohistochemistry of Nephrin in mouse kidney tissue with Nephrin antibody at 1ug/ml.

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  Immunohistochemistry of Nephrin in rat kidney tissue with Nephrin antibody at 5ug/ml.

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  Immunofluorescence of Nephrin in Mouse Kidney cells with Nephrin antibody at 10ug/ml.

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  Immunofluorescence of Nephrin in rat kidney tissue with Nephrin antibody at 20ug/ml.

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Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1-4 µg/mL
  IHC-P: 1-2 µg/mL.
  
  Antibody validated: Western Blot in human, mouse and rat samples
  Immunohistochemistry in mouse, and rat samples. All other applications and species not yet tested.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Anti-Nephrin antibody (2265) was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human Nephrin.
  The immunogen is located within the last 50 amino acids of Nephrin.
• Formulation: Nephrin Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• Purification: Nephrin Antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated
• Storage: Nephrin antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: Predicted: 135 kD + 10 N-linked glycosylation sites
  
  Observed: 200 kD
• Gene Name: NPHS1 nephrin
• Database Link:
  NP_004637
  Entrez Gene 54631 MouseEntrez Gene 64563 RatEntrez Gene 4868 Human
  UniProt ID O60500
• Background: Nephrin Antibody: Nephrin is strongly expressed in renal glomeruli and is a member of the immunoglobulin family of cell adhesion molecules. Mutations in the Nephrin gene result in congenital nephrotic syndrome, an autosomal-recessive disorder characterized by massive proteinuria in utero and nephrosis at birth. Renal glomeruli allow normal kidneys to filter plasma so that it is very pure. Nephrin is expressed in the podocyte slit-diaphragm of the renal glomeruli in a manner that suggests that Nephrin molecules homodimerize in an anti-parallel fashion similar to cadherin interactions in adherens junctions. Thus, Nephrin may constitute the entire extracellular structure of the slit-diaphragm.
• Synonyms: CNF; nephrin; NPHN
• Note: Optimal dilutions for each application to be determined by the researcher.

Reference Data

• Protein Families: Druggable Genome, Transmembrane