Nephrin (NPHS1) Rabbit Polyclonal Antibody

CAT#: TA306037

Rabbit Polyclonal Nephrin Antibody

Product Images

Western blot analysis of Nephrin in mouse kidney tissue lysate with Nephrin antibody at 1ug/ml in the (A) absence and (B) presence of blocking peptide.

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  Figure from citation: Western Blot of NPHS1 (Nephrin) protein level by using anti-NPHS1 antibody in cell lysates of quiescent mouse podocytes. View Citation

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  Immunohistochemistry of Nephrin in mouse kidney tissue with Nephrin antibody at 1ug/ml.

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  Immunohistochemistry of Nephrin in rat kidney tissue with Nephrin antibody at 5ug/ml.

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  Immunofluorescence of Nephrin in Mouse Kidney cells with Nephrin antibody at 10ug/ml.

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  Immunofluorescence of Nephrin in rat kidney tissue with Nephrin antibody at 20ug/ml.

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Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1 ug/mL, ICC: 1 ug/mL, IF: 10 ug/mL
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Nephrin antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human Nephrin. The immunogen is located within the last 50 amino acids of Nephrin.
• Formulation: PBS containing 0.02% sodium azide.
• Purification: Affinity chromatography purified via peptide column
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: NPHS1 nephrin
• Database Link:
  NP_004637
  Entrez Gene 54631 MouseEntrez Gene 64563 RatEntrez Gene 4868 Human
  UniProt ID O60500
• Background: Nephrin is strongly expressed in renal glomeruli and is a member of the immunoglobulin family of cell adhesion molecules. Mutations in the Nephrin gene result in congenital nephrotic syndrome, an autosomal-recessive disorder characterized by massive proteinuria in utero and nephrosis at birth. Renal glomeruli allow normal kidneys to filter plasma so that it is very pure. Nephrin is expressed in the podocyte slit-diaphragm of the renal glomeruli in a manner that suggests that Nephrin molecules homodimerize in an anti-parallel fashion similar to cadherin interactions in adherens junctions. Thus, Nephrin may constitute the entire extracellular structure of the slit-diaphragm. Despite its predicted molecular weight, Nephrin often migrates at a lower than expected size in SDS-PAGE.
• Synonyms: CNF; nephrin; NPHN

Reference Data

• Protein Families: Druggable Genome, Transmembrane