IKAP (IKBKAP) Rabbit Polyclonal Antibody

CAT#: TA306056

Rabbit Polyclonal IKAP Antibody

Product Images

Western blot analysis of IKAP in A-20 cell lysate with IKAP antibody at in (A) 0.5, and (B) 1 ug/mL.

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  Immunocytochemistry of IKAP in A-20 cells with IKAP antibody at 1 ug/mL.

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  Immunofluorescence of IKAP in A20 cells with IKAP antibody at 20 ug/mL.

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: WB: 0.5 - 1 ug/mL, ICC: 1 ug/mL, IF: 20 ug/mL
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: IKAP antibody was raised against a 16 amino acid peptide from near the carboxy terminus of human IKAP.
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1ug/ul
• Purification: Affinity chromatography purified via peptide column
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase complex-associated protein
• Database Link:
  NP_003631
  Entrez Gene 230233 MouseEntrez Gene 8518 Human
  UniProt ID O95163
• Background: IKAP was initially identified as a scaffold protein of the IkappaB kinase complex that could bind to IKKalpha, IKKbeta, NF-kappaB, and the NF-kappaB-inducing kinase (NIK), although later evidence has cast doubt on this. More recent reports show that mutations in IKAP such as a frameshift leading to a truncated protein or a missense mutation that leads to defective phosphorylation are responsible for the autosomal recessive genetic disease familial dysautonomia (FD). Reports indicating that it forms part of the RNA polymerase II transcription elongation complex suggest that this disease may be due to compromised transcription elongation. More recently, it was shown that IKAP associates with c-Jun N-terminal kinase (JNK) and could specifically enhance JNK activation induced by the upstream JNK activators MEKK1 and ASK1, indicating another possible cause for FD. At least two isoforms of IKAP are known two exist.
• Synonyms: DYS; ELP1; FD; IKAP; IKI3; TOT1

Reference Data

• Protein Families: Druggable Genome