DIABLO Rabbit Polyclonal Antibody

CAT#: TA306067

Rabbit Polyclonal Smac Antibody

Product Images

Western blot analysis of Smac in mouse heart tissue lysate in the absence (A) or presence (B) of blocking peptide and in rat heart tissue lysate with Smac antibody at 1 ug /ml.

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  Immunohistochemical staining of mouse spleen tissue using mSmac antibody at 2 ug/ml.

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  Immunofluorescence of Smac in Mouse Spleen cells with Smac antibody at 10 ug/mL.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1 ug/mL, ICC: 2 ug/mL, IF: 10 ug/mL
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Smac antibody was raised against a peptide corresponding to amino acids 222 to 237 of murine Smac/DIABLO.
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1ug/ul
• Purification: Affinity chromatography purified via peptide column
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: diablo IAP-binding mitochondrial protein
• Database Link:
  NP_063940
  Entrez Gene 66593 MouseEntrez Gene 288753 RatEntrez Gene 56616 Human
  UniProt ID Q9NR28
• Background: The inhibitor of apoptosis proteins (IAPs) regulate programmed cell death by inhibiting members of the caspase family of enzymes. A novel mammalian protein that binds to IAPs and neutralizes the inhibitory effect of IAPs on caspases was recently identified and designated Smac/DIABLO (1,2). Smac/DIABLO is a mitochondrial protein that is released along with cytochrome c during apoptosis and activates cytochrome c/Apaf-1/capase-9 pathway. Analysis of the structural basis of Smac/DIABLO reveals that the N-terminal amino acids are required for binding of Smac/DIABLO to IAPs and activation of caspases (3-6). Smac/DIABLO is expressed in a variety of human and mouse tissues (1,2).
• Synonyms: DFNA64; SMAC

Reference Data

• Protein Families: Transmembrane