Endo G (ENDOG) Rabbit Polyclonal Antibody

CAT#: TA306105

Rabbit Polyclonal EndoG Antibody

Product Images

Western blot analysis of EndoG in mouse (M) 3T3 and human (H) HepG2 cell lysates with EndoG antibody at 2ug/ml.

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  Immunohistochemistry of EndoG in human pancreas with EndoG antibody at 15ug/ml.

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  Immunofluorescence of EndoG in human pancreas tissue with EndoG antibody at 20ug/ml.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 2 ug/mL, ICC: 15 ug/mL
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: EndoG antibody was raised with a synthetic peptide corresponding to 15 amino acids near the amino terminus of human EndoG. The immunogen is located within amino acids 40 - 90 of EndoG.
• Formulation: PBS containing 0.02% sodium azide.
• Purification: Affinity chromatography purified via peptide column
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: endonuclease G
• Database Link:
  NP_004426
  Entrez Gene 13804 MouseEntrez Gene 362100 RatEntrez Gene 2021 Human
  UniProt ID Q14249
• Background: The fragmentation of nuclear DNA is a hallmark of apoptotic cell death. The activities of caspase and nuclease are involved in the DNA fragmentation. Caspase-activated deoxyribonuclease (CAD), also termed DNA fragmentation factor (DFF40), is one such nuclease, and is capable of inducing DNA fragmentation and chromatin condensation after cleavage by caspase-3 of its inhibitor ICAD/DFF45. Caspase and CAD independent DNA fragmentation also exists. Recent studies demonstrated that another nuclease, endonuclease G (endoG), is specifically activated by apoptotic stimuli and is able to induce nucleosomal fragmentation of DNA independently of caspase and DFF/CAD (1,2). EndoG is a mitochondrion-specific nuclease that translocates to the nucleus and cleaves chromatin DNA during apoptosis. The homologue of mammalian EndoG is the first mitochondrial protein identified to be involved in apoptosis in C. elegans (2). EndooG also cleaves DNA in vitro (4).
• Synonyms: FLJ27463

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Apoptosis