Bcl2 Binding component 3 (BBC3) Rabbit Polyclonal Antibody

CAT#: TA306109

Rabbit Polyclonal PUMA Antibody

Product Images

Western blot analysis of PUMA expression in K562 cell lysate with PUMA antibody at 2 ug /ml.

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  Immunohistochemistry of PUMA in human breast carcinoma with PUMA antibody at 10 ug/ml.

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  Immunofluorescence of PUMA in K562 cells with PUMA antibody at 10 ug/mL.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 2 ug/mL, IHC: 10 ug/mL, IF: 10 ug/mL
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: PUMA antibody was raised against a synthetic peptide corresponding to 14 amino acids near the amino terminus of human PUMA-a This sequence is identical between a and b ?forms of the PUMA proteins.
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1ug/ul
• Purification: Affinity chromatography purified via peptide column
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: BCL2 binding component 3
• Database Link:
  NP_055232
  Entrez Gene 27113 Human
  UniProt ID Q96PG8
• Background: Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse (1-3). PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-alpha and PUMA-beta (1). PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.
• Synonyms: JFY-1; JFY1; PUMA

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Huntington's disease, p53 signaling pathway