IRAK4 Rabbit Polyclonal Antibody

CAT#: TA306123

Rabbit Polyclonal IRAK-4 Antibody

Product Images

Western blot analysis of IRAK-4 in HeLa cell lysate with IRAK-4 antibody at 1 (lane 1), 2 (lane 2), and 4 (lane 3) ug/mL, respectively.

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  Immunocytochemistry of IRAK-4 in K562 cells with IRAK-4 antibody at 10 ug/mL.

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  Immunofluorescence of IRAK-4 in K562 cells with IRAK-4 antibody at 10 ug/mL.

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: WB: 1 - 4 ug/mL, ICC: 10 ug/mL, IF: 10 ug/mL
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: IRAK-4 antibody was raised against a synthetic peptide corresponding to amino acids at the carboxy terminus of human IRAK-4.
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1ug/ul
• Purification: Affinity chromatography purified via peptide column
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: interleukin 1 receptor associated kinase 4
• Database Link:
  NP_001107654
  Entrez Gene 51135 Human
  UniProt ID Q9NWZ3
• Background: Interleukin-1 (IL-1) and lipopolysaccharide (LPS) induces cellular responses through IL-1 receptor (IL-1R) and Toll-like receptors (TLR). IL-1R-associated kinases (IRAK, IRAK2, and IRAK-M) regulate the activation of NF-kappaB and MAP kinase (MAPK) by IL-1R/TLR (1-3). A novel member in the IRAK/Pelle family was recently identified and designated IRAK-4 (4,5). Overexpression of IRAK-4 activates NF-kB and MAPK pathways. IRAK-4 interacts with and phosphorylates IRAK-1. IRAK-4-deficient animals are completely resistant to the challenge with LPS. Animals and humans lacking IRAK-4 are impaired in their responses to viral and bacterial challenges (5,6). Members in IRAK/Pelle family play a central role in IL-1R/TLR mediated inflammatory responses and in innate immunity.
• Synonyms: IPD1; IRAK-4; NY-REN-64; REN64

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Apoptosis, Neurotrophin signaling pathway, Toll-like receptor signaling pathway