DAD1 Rabbit Polyclonal Antibody

CAT#: TA306165

Rabbit Polyclonal DAD1 Antibody

Product Images

Western blot analysis of DAD1 in HepG2 cell lysate with DAD1 antibody at (A) 0.5, (B) 1, and (C) 2 ug/mL.

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  Immunocytochemistry of DAD1 in HepG2 cells with DAD1 antibody at 10 ug/mL.

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  Immunofluorescence of DAD1 in HepG2 cells with DAD1 antibody at 10 ug/mL.

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: WB: 0.5 - 2 ug/mL, ICC: 10 ug/mL, IF: 10 ug/mL
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: DAD1 antibody was raised against a peptide corresponding to 14 amino acids near the C-terminus of human DAD1.
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1ug/ul
• Purification: Ion exchange chromatography purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: defender against cell death 1
• Database Link:
  NP_001335
  Entrez Gene 13135 MouseEntrez Gene 1603 Human
  UniProt ID P61803
• Background: Defender of cell death 1 (DAD1) was initially discovered in BHK21 cells as a negative regulator of programmed cell death (1), a process important for normal organism development and tissue homeostasis (2). DAD1 was later shown to be a subunit of the mammalian oligosaccharyltransferase complex and is required for its function and structural integrity (3,4). Mice lacking DAD1 express abnormal N-linked glycoproteins and undergo increased apoptotic-associated embryonic death (5,6). Furthermore, overexpression of DAD1 mRNA is seen in some human hepatocellular carcinomas (7), indicating it may also play a role in carcinogenesis. It should be noted that DAD1 is not related to the inhibitor of apoptosis proteins (IAP) family and does not contain any baculoviral IAP repeat (BIR) domains.
• Synonyms: OST2

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Metabolic pathways, N-Glycan biosynthesis