BAD Rabbit Polyclonal Antibody

CAT#: TA306174

Rabbit Polyclonal BAD Antibody

Product Images

Western blot analysis of Bad in T24 cell lysates with Bad antibody at (A) 0.5, (B) 1, and (C) 2 ug/ml.

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  Immunohistochemical staining of rat thymus using anti-Bad at 2 ug/ml.

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  Immunofluorescence of BAD in Rat Thymus cells with BAD antibody at 10 ug/mL.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 0.5 - 2 ug/mL, ICC: 2 ug/mL, IF: 10 ug/mL
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Bad antibody was raised against a peptide corresponding to 15 amino acids near the C-terminus of human Bad.
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1ug/ul
• Purification: Ion exchange chromatography purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: BCL2 associated agonist of cell death
• Database Link:
  NP_004313
  Entrez Gene 12015 MouseEntrez Gene 64639 RatEntrez Gene 572 Human
  UniProt ID Q92934
• Background: Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death (reviewed in 1 and 2). Bcl-2 homology 3 (BH3) domain containing pro-apoptotic proteins, such as Bax, Bid, and Bik, form a growing subclass of the Bcl-2 family. Another such protein is the Bcl-2-antagonist of cell death (Bad). Bad regulates apoptosis by forming heterodimers with anti-apoptotic proteins Bcl-2 and Bcl-xL, thereby preventing them from binding with Bax (3). Bad activity is regulated by its phosphorylation; it is inactivated by kinases such as Akt and MAP kinase and thus promotes cell survival (4), whereas JNK-induced phosphorylation promotes the apoptotic role of Bad (5).
• Synonyms: BBC2; BCL2L8

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Acute myeloid leukemia, Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Apoptosis, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, ErbB signaling pathway, Focal adhesion, Insulin signaling pathway, Melanoma, Neurotrophin signaling pathway, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Prostate cancer, VEGF signaling pathway