UBE2N Rabbit Polyclonal Antibody

CAT#: TA306183

Rabbit Polyclonal UBC13 Antibody

Product Images

Western blot analysis of UBC13 in human small intestine cell lysates with UBC13 antibody at (A) 0.5, (B) 1, and (C) 2 ug/ml.

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  Immunohistochemistry of UBC13 in mouse thymus tissue with UBC13 antibody at 2 ug/ml.

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  Immunofluorescence of UBC13 in Mouse Thymus cells with UBC13 antibody at 10 ug/mL.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 0.5 - 2 ug/mL, ICC: 2 ug/mL, IF: 10 ug/mL
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: UBC13 antibody was raised against a peptide corresponding to 15 amino acids near the C-terminus of human UBC13.
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1ug/ul
• Purification: Ion exchange chromatography purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: ubiquitin conjugating enzyme E2 N
• Database Link:
  NP_003339
  Entrez Gene 93765 MouseEntrez Gene 116725 RatEntrez Gene 7334 Human
  UniProt ID P61088
• Background: Ubiquitin-conjugating enzyme 13 (Ubc13) was initially discovered in S. cerevisiae as a DNA-damage inducible protein involved in the error-free DNA postreplication repair pathway (1). It has recently been shown to be an important component of the Toll-like receptor and IL-1R signaling pathway (reviewed in 2). Signals from these pathways are relayed by a number of downstream molecules such as MyD88 and tumor necrosis factor receptor associated factor (TRAF6), ultimately activating various kinases and transcription factors (2,3). Ubc13 is part of a dimeric ubiquitin-conjugating enzyme complex also containing UEV1A (ubiquitin-conjugating enzyme E2 variant 1) that together with TRAF6 activates TAK1, a member of the mitogen-activated protein kinase kinase kinase family (4-6). The Ubc13-UEV1A complex also mediates the Lys-63 ubiquitination of TRAF-6, and this ubiquitination is essential for TAK1 activation (5).
• Synonyms: HEL-S-71; UBC13; UbcH-ben; UbcH13; UBCHBEN

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Ubiquitin mediated proteolysis