ECSIT Rabbit Polyclonal Antibody

CAT#: TA306185

Rabbit Polyclonal ECSIT Antibody

Product Images

Western blot analysis of ECSIT in human heart cell lysates with ECSIT antibody at (A) 0.5, (B) 1, and (C) 2 ug/ml.

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  Immunohistochemistry of ECSIT in mouse heart cells with ECSIT antibody at 2 ug/ml.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 0.5 - 2 ug/mL, ICC: 2 ug/mL
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: ECSIT antibody was raised against a peptide corresponding to 14 amino acids near the C-terminus of human ECSIT.
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1ug/ul
• Purification: Ion exchange chromatography purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: ECSIT signalling integrator
• Database Link:
  NP_001135936
  Entrez Gene 26940 MouseEntrez Gene 51295 Human
  UniProt ID Q9BQ95
• Background: Activation of NF-?B as a result of Toll-like receptor (TLR) and IL-1 receptor signaling is a major component of innate immune responses (reviewed in 1). Signals from these receptors are relayed by a number of adapter molecules such as TRIF, TIRAP, and MyD88 (2) to kinases such as IRAK (3) and other intermediates such as TNF receptor associated factor (TRAF)-6 (4). ECSIT (evolutionarily conserved signaling intermediate in Toll pathways) was initially identified as a cytoplasmic protein interacting specifically with TNF receptor associated factor (TRAF)-6 in the TLR pathway (5). Recently however, ECSIT has also been shown to be required for bone morphogenetic protein (Bmp) signaling and mesoderm formation during mouse embryogenesis (6), indicating the possibility of cross-talk between the TLR/IL-B and Bmp signaling pathways.
• Synonyms: SITPEC

Reference Data

• Protein Pathways: MAPK signaling pathway