IRF3 Rabbit Polyclonal Antibody

CAT#: TA306189

Rabbit Polyclonal IRF3 Antibody

Product Images

Western blot analysis of IRF3 in Ramos whole cell lysate with IRF3 antibody at (A) 1, (B) 2, and (C) 4 ug/ml.

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  Immunohistochemical staining of mouse kidney using IRF3 antibody at 2 ug/ml.

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  Immunofluorescence of IRF3 in Mouse Kidney cells with IRF3 antibody at 20 ug/mL.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1 - 4 ug/mL, ICC: 2 ug/mL, IF: 20 ug/mL
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: IRF3 antibody was raised against a peptide corresponding to 14 amino acids near the carboxy-terminus of human IRF3.
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1ug/ul
• Purification: Affinity chromatography purified via peptide column
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: interferon regulatory factor 3
• Database Link:
  NP_001562
  Entrez Gene 54131 MouseEntrez Gene 3661 Human
  UniProt ID Q14653
• Background: Interferons (IFN)s are involved in a multitude of immune interactions during viral infections and play a major role in both the induction and regulation of innate and adaptive antiviral mechanisms (1 for review). During infection, host-virus interactions signal downstream molecules such as transcription factors such as IFN regulatory factor-3 (IRF3) which can act to stimulate transcription of IFN-alpha/beta genes. IRF3 is present in an inactive form in the cytoplasm of most cells (2). Following viral infection, IRF3 can be activated by IkappaB kinase-epsilon and TANK-binding kinase 1 (TBK1) (3,4), whereupon IRF3 translocates to the nucleus. IRF3 can also be activated by stimulation of toll-like receptor 3 (TLR3) by dsRNA (3,5). IRF3 exists as at least two distinct isoforms.
• Synonyms: interferon regulatory factor 3; MGC94729

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Cytosolic DNA-sensing pathway, RIG-I-like receptor signaling pathway, Toll-like receptor signaling pathway