IRF3 Rabbit Polyclonal Antibody

CAT#: TA306191

Rabbit Polyclonal IRF8 Antibody

Product Images

Western blot analysis of IRF8 in human thymus tissue lysate with IRF8 antibody at 1ug/ml in (A) the presence and (B) absence of blocking peptide.

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  Immunofluorescence of IRF8 in K562 cells with IRF8 antibody at 10 ug/mL.

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: WB: 1 ug/mL, IF: 10 ug/mL
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: IRF8 antibody was raised against a peptide corresponding to 16 amino acids near the carboxy-terminus of human IRF8.
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1ug/ul
• Purification: Affinity chromatography purified via peptide column
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: interferon regulatory factor 3
• Database Link:
  NP_001562
  Entrez Gene 54131 MouseEntrez Gene 292892 RatEntrez Gene 3661 Human
  UniProt ID Q14653
• Background: Interferons (IFN)s are involved in a multitude of immune interactions during viral infections and play a major role in both the induction and regulation of innate and adaptive antiviral mechanisms (1 for review). During infection, host-virus interactions signal downstream molecules such as transcription factors such as IFN regulatory factor-3 (IRF3) which can act to stimulate transcription of IFN-alpha/beta genes. Unlike IRF3, IRF8 appears to act as a negative regulator of IFN-induced genes in most cases (2,3), but IRF8 mediates activation of NF-kappaB by the toll-like receptor 9 (TLR9) after stimulation by unmethylated CpG DNA in dendritic cells (4,5). Finally, it has been shown that IRF8 decreases bcl-2 expression and thus may play a role in chronic myelogenous leukemia (6).
• Synonyms: interferon regulatory factor 3; MGC94729

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Cytosolic DNA-sensing pathway, RIG-I-like receptor signaling pathway, Toll-like receptor signaling pathway