RHEB Rabbit Polyclonal Antibody

CAT#: TA306215

Rabbit Polyclonal Rheb Antibody

Product Images

Western blot analysis of Rheb in rat heart cell lysate with Rheb antibody at (A) 2 and (B) 4 ug/ml.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 2 - 4 ug/mL
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Rabbit Rheb polyclonal antibody was raised against a 14 amino acid peptide from the amino terminus of human Rheb.
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1ug/ul
• Purification: Affinity chromatography purified via peptide column
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Ras homolog enriched in brain
• Database Link:
  NP_005605
  Entrez Gene 19744 MouseEntrez Gene 26954 RatEntrez Gene 6009 Human
  UniProt ID Q15382
• Background: Rheb (Ras homolog enriched in brain) is an evolutionarily conserved member of the Ras family of small GTP-binding proteins originally found to be rapidly induced by synaptic activity in the hippocampus following seizure (1). While it is expressed at relatively high levels in the brain, Rheb is widely expressed in other tissues and may be induced by growth factor stimulation. Similar to other family members, Rheb triggers activation of the Raf-MEK-MAPK pathway (2). Biochemical and genetic studies demon-strate that Rheb has an important role in regulating the insulin/Target of rapamycin (TOR) signaling pathway (3-5). TOR is a serine/threonine protein kinase that acts as a sensor for ATP and amino acids, balancing the availability of nutrients with protein translation and cell growth. A dimeric protein complex termed TSC1/TSC2 indirectly inhibits TOR activity by inhibiting Rheb via the GAP activity of TSC2 (3).
• Synonyms: RHEB2

Reference Data

• Protein Pathways: Insulin signaling pathway, mTOR signaling pathway