Hamartin (TSC1) Rabbit Polyclonal Antibody

CAT#: TA306218

Rabbit Polyclonal TSC1 Antibody

Product Images

Western blot analysis of TSC1 in EL4 cell lysate with TSC1 antibody at 1 ug/mL in the (A) absence and (B) presence of blocking peptide.

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  Immunocytochemistry of TSC1 in EL4 cells with TSC1 antibody at 2 ug/mL

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  Immunofluorescence of TSC1 in L1210 cells with TSC1 antibody at 10 ug/mL.

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: WB: 1 ug/mL, ICC: 2 ug/mL, IF: 2 ug/mL
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: TSC1 antibody was raised against a 15 amino acid peptide from the middle region of human TSC1. TSC1 antibody will recognize both isoforms of TSC1.
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1ug/ul
• Purification: Affinity chromatography purified via peptide column
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: tuberous sclerosis 1
• Database Link:
  NP_000359
  Entrez Gene 60445 RatEntrez Gene 64930 MouseEntrez Gene 7248 Human
  UniProt ID Q92574
• Background: Tuberous sclerosis complex (TSC) is an autosomal dominant tumor syndrome caused by mutations in either of the TSC1 or TSC2 tumor suppressor genes. The products of these genes form a protein complex that indirectly decreases the signaling of the mammalian Target of Rapamycin (TOR), an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors (reviewed in 1,2). TOR activity is stimulated by Rheb, a member of the Ras superfamily of G-proteins, when the GTP/GDP ratio bound to Rheb is high (3). Immunoprecipitated TSC1/TSC2 has been shown to stimulate Rheb GTPase activity in vitro (4), suggesting that the TSC1/TSC2 decreases the ability of Rheb to stimulate TOR activity. This is supported by experiments showing overexpression of TSC1 and TSC2 results in a significant decrease in the GTP/GDP ratio bound to Rheb and the inhibition of cell growth (4,5). A shorter 40 kDa isoform of TSC1 has been shown to exist but its function is unknown.
• Synonyms: LAM; TSC

Reference Data

• Protein Pathways: Insulin signaling pathway, mTOR signaling pathway