LAMP2 Rabbit Polyclonal Antibody

CAT#: TA306261

Rabbit Polyclonal LAMP-2 Antibody

Product Images

Western blot analysis of LAMP-2 in HepG2 cell lysate with LAMP-2 antibody at (A) 1 and (B) 2ug/ml.

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  Immunohistochemistry of LAMP-2 in mouse liver tissue with LAMP-2 Antibody at 5ug/ml.

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  Immunocytochemistry of LAMP-2 in HepG2 cells with LAMP-2 antibody at 10ug/ml.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1 - 2 ug/mL, ICC: 10 ug/mL
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: LAMP-2 antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human LAMP-2. The immunogen is located within the last 50 amino acids of LAMP-2.
• Formulation: PBS containing 0.02% sodium azide.
• Purification: Affinity chromatography purified via peptide column
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: lysosomal associated membrane protein 2
• Database Link:
  NP_001116078
  Entrez Gene 16784 MouseEntrez Gene 3920 Human
  UniProt ID P13473
• Background: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components (1,2) and is negatively regulated by TOR (Target of rapamycin) (3). LAMP-2, a highly glycosylated protein associated with the lysosome (4), has recently been shown to be important in autophagy as mice deficient in this protein failed to convert autophagic vacuoles into vacuoles (5) leading to impaired degradation of long-lived proteins. This correlates with the finding that human LAMP-2 deficiency causing Danon’s disease is associated with the accumulation of autophagic material in striated myocytes (6). LAMP-2 exists in multiple isoforms (7).
• Synonyms: CD107b; LAMP-2; LAMPB; LGP110

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Lysosome