TLR5 Rabbit Polyclonal Antibody

CAT#: TA306270

Rabbit Polyclonal TLR5 Antibody

Product Images

Western blot analysis of TLR5 expression in HT29 cell lysate with TLR5 antibody at (A) 1 and (B) 2ug/ml.

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  Immunofluorescence of TLR5 in THP1 cells with TLR5 antibody at 10ug/ml.

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  Immunocytochemistry of TLR5 in THP-1 cells with TLR5 antibody at 10ug/ml.

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: WB: 0.5 - 2 ug/mL, ICC: 10 ug/mL, IF: 10 ug/mL
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: TLR5 antibody was raised against a peptide corresponding to 15 amino acids near the carboxy terminus of human TLR5. The immunogen is located within the last 50 amino acids of TLR5.
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1ug/ul
• Purification: Affinity chromatography purified via peptide column
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: toll like receptor 5
• Database Link:
  NP_003259
  Entrez Gene 53791 MouseEntrez Gene 7100 Human
  UniProt ID O60602
• Background: Toll-like receptors (TLRs) are evolutionarily conserved pattern-recognition molecules resembling the toll proteins that mediate antimicrobial responses in Drosophila. These proteins recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses (1,2). The TLRs act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors (3) so the organism can respond to potential infection. TLR5 recognizes flagellin from both Gram-positive and Gram-negative bacteria and will cause the activation of NF-kappaB, leading to the activation of TNF-Α and other cytokines (4). A common TLR5 stop codon polymorphism that disrupts TLR5 signaling is associated with susceptibility to Legionnaires’ disease and demonstrates the importance of TLR5 in the innate immune response (5).
• Synonyms: MELIOS; SLE1; SLEB1; TIL3

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Pathogenic Escherichia coli infection, Toll-like receptor signaling pathway