XBP1 Rabbit Polyclonal Antibody

CAT#: TA306289

Rabbit Polyclonal XBP-1 Antibody

Product Images

Western blot analysis of XBP-1 in HepG2 cell lysate with XBP-1 antibody at (A) 0.5, (B) 1 and (C) 2ug/ml.

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  Western blot analysis of XBP1 expression in rat liver tissue lysate with XBP1 antibody at (A) 1 and (B) 2ug/ml.

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  Immunohistochemistry of XBP1 in human liver tissue with XBP1 antibody at 5ug/ml.

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  Immunohistochemistry of XBP1 in mouse liver tissue with XBP1 antibody at 2ug/ml.

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  Immunohistochemistry of XBP-1 in rat liver tissue with XBP-1 Antibody at 5ug/ml.

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  Immunofluorescence of XBP-1 in HepG2 cells with XBP-1 antibody at 20ug/ml.

• Immunofluorescence of XBP1 in human liver tissue with XBP1 antibody at 20ug/ml.

• Immunofluorescence of XBP-1 in rat liver tissue with XBP-1 Antibody at 20ug/ml.

• Immunocytochemistry of XBP-1 in HepG2 cells with XBP-1 antibody at 10ug/ml.

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Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 0.5 - 2 ug/mL, ICC: 10 ug/mL
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: XBP-1 antibody was raised against an 18 amino acid synthetic peptide from near the amino terminus of human XBP-1. The immunogen is located within amino acids 40 - 90 of XBP-1.
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1ug/ul
• Purification: Affinity chromatography purified via peptide column
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: X-box binding protein 1
• Database Link:
  NP_001073007
  Entrez Gene 22433 MouseEntrez Gene 7494 Human
  UniProt ID P17861
• Background: X box binding protein 1 (XBP-1) is a key protein in the mammalian unfolded protein response (UPR) that protects the cell against the stress of malfolded proteins in the endoplasmic reticulum (ER) . Upon sensing unfolded proteins, an ER transmembrane endonuclease and kinase termed IRE1p is activated and excises an intron from XBP-1 mRNA. The spliced XBP-1 mRNA results in a 371 amino acid protein (XBP-1s) which is then translocated to the nucleus where it binds to the regulatory elements of downstream genes. Together with other UPR transcription factors such as ATF6, XBP-1 stimulates the production of ER stress proteins including the ER resident protein chaperones glucose regulated protein (GRP) 78 and GRP94 (3,4).
• Synonyms: TREB-5; TREB5; XBP-1; XBP2

Reference Data

• Protein Families: Transcription Factors