DDX3 (DDX3X) Rabbit Polyclonal Antibody

CAT#: TA306299

Rabbit Polyclonal DDX3 Antibody

Product Images

Western blot analysis of DDX3 in HepG2 cell lysate with DDX3 antibody at (A) 0.5 and (B) 1 ug/mL.

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  Immunocytochemistry of DDX3 in HepG2 cells with DDX3 antibody at 10 ug/mL.

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  Immunofluorescence of DDX3 in HepG2 cells with DDX3 antibody at 20 ug/mL.

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: WB: 0.5 - 1 ug/mL, ICC: 10 ug/mL, IF: 20 ug/mL
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: DDX3 antibody was raised against a 16 amino acid peptide from near the amino terminus of human DDX3.
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1ug/ul
• Purification: Affinity chromatography purified via peptide column
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: DEAD-box helicase 3, X-linked
• Database Link:
  NP_001347
  Entrez Gene 13205 MouseEntrez Gene 1654 Human
  UniProt ID O00571
• Background: DDX3 contains all of the motifs of the DEAD-box family of RNA helicases, including the Asp-Glu-Ala-Asp sequence that gives the protein family its name and distinguishes it from other RNA helicases (1,2). DDX3 is localized to the X chromosome and has a highly conserved functional homolog (DBY) on the Y chromosome (3). DDX3 is thought to be involved in RNA splicing, RNA transport, and translation initiation (4). It has also been shown to be involved in cell growth control and is deregulated in hepatitis virus-associated hepatocellular carcinoma (5). Recent experiments suppressing DDX3 expression blocked HIV-1 RNA export from the nucleus, suggesting that DDX3 functions as a shuttling protein that transports the HIV-1 protein Rev and its cofactor CRM1 from the nucleus to the cytoplasm (6).
• Synonyms: CAP-Rf; DBX; DDX3; DDX14; HLP2

Reference Data

• Protein Families: ES Cell Differentiation/IPS
• Protein Pathways: RIG-I-like receptor signaling pathway