KDM1A Rabbit Polyclonal Antibody

CAT#: TA306302

Rabbit Polyclonal LSD1 Antibody

Product Images

Western blot analysis of LSD1 in P815 cell lysate with LSD1 antibody at (A) 1 and (B) 2 ug/ml.

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  Immunohistochemistry of LSD1 in human small intestine tissue with LSD1 antibody at 2 ug/ml.

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  Immunofluorescence of LSD1 in Human Small Intestine cells with LSD1 antibody at 20 ug/mL.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1 - 2 ug/mL, ICC: 2 ug/mL, IF: 20 ug/mL
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: LSD1 antibody was raised against a 16 amino acid peptide from near the center of human LSD1.
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1ug/ul
• Purification: Affinity chromatography purified via peptide column
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: lysine demethylase 1A
• Database Link:
  NP_001009999
  Entrez Gene 99982 MouseEntrez Gene 500569 RatEntrez Gene 23028 Human
  UniProt ID O60341
• Background: Histone modifications mediate changes in gene expression by altering chromatin structure or by serving as a platform to recruit other proteins. LSD1 is a recently discovered amine oxidase that catalyzes the lysine-specific demethylation of histone proteins via an FAD-dependent oxidative reaction (1). Methylation on histone H3-K9 is thought to play an important role in heterochromatin formation, while methylation on arginine and some lysine residues (such as H3-K4) is associated with active transcription (2). LSD1 associates with various proteins, including HDAC1/2, CoREST, and BHC80, that act to regulate LSD1 activity in vivo, and in a histone H3-K4-specific methylase complex that is involved in transcriptional regulation (3,4). Experiments have shown that CoREST, a SANT domain-containing corepressor (5) acts to enhance LSD1 activity, while BHC80, a PHD domain-containing protein (6), inhibits CoREST/LSD1 activity in vitro (3). LSD1-mediated histone demethylation thus may have significant effects on gene expression.
• Synonyms: AOF2; BHC110; CPRF; KDM1; LSD1

Reference Data

• Protein Families: Druggable Genome, Transcription Factors