HRK Rabbit Polyclonal Antibody

CAT#: TA306305

Rabbit Polyclonal Hrk Antibody

Product Images

Western blot analysis of Hrk in mouse pancreas tissue lysate with Hrk antibody at (A) 2.5 and (B) 5 ug/ml.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 2.5 - 5 ug/mL
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: HRK antibody was raised against a 15 amino acid peptide from near the center of human Hrk.
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1ug/ul
• Purification: Affinity chromatography purified via peptide column
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: harakiri, BCL2 interacting protein
• Database Link:
  NP_003797
  Entrez Gene 12123 MouseEntrez Gene 8739 Human
  UniProt ID O00198
• Background: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Hrk, a pro-apoptotic member of the Bcl-2 homology domain-3 (BH3)-only group of the Bcl-2 family of proteins, was also identified as novel protein induced during programmed neuronal death. It lacks significant homology to other Bcl-2 family members except for an 8-amino acid region that is similar to the BH3 motif of Bik. Hrk regulates apoptosis through interaction with the anti-apoptotic proteins Bcl-2 and Bcl-XL via this domain. It does not interact with the pro-apoptotic proteins Bax, Bak, or Bcl-XS. Hrk localizes to mitochondrial membranes in a pattern similar to that previously reported for Bcl-2 and Bcl-XL. Despite its predicted molecular weight, Hrk often migrates at 12-15 kDa.
• Synonyms: DP5; HARAKIRI

Reference Data

• Protein Families: Druggable Genome, Transmembrane