Bif (SH3GLB1) Rabbit Polyclonal Antibody

CAT#: TA306324

Rabbit Polyclonal Bif Antibody

Product Images

Western blot analysis of BIF in HeLa cell lysate with BIF antibody at (A) 1 and (B) 2 ug/mL.

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  Immunocytochemistry of BIF in HeLa with BIF antibody at 10 ug/mL.

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: WB: 1 - 2 ug/mL, ICC: 10 ug/mL
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: BIF antibody was raised against a 15 amino acid peptide from near the carboxy terminus of human BIF.
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1ug/ul
• Purification: Affinity chromatography purified via peptide column
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: SH3 domain containing GRB2 like endophilin B1
• Database Link:
  NP_057093
  Entrez Gene 54673 MouseEntrez Gene 51100 Human
  UniProt ID Q9Y371
• Background: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells and is caused by the activation of proteolytic enzymes termed caspases. Proteins that comprise the Bcl-2 family such as Bax appear to control the activation of these enzymes. Bax activity was found to be regulated by its association with Bax-interacting factor 1 (BIF), a member of the endophilin B family that is associated with intracellular membranes.. Following this interaction, Bax undergoes a conformational change and translocates to mitochondrial membranes. The Bax/BIF interaction appears to be enhanced by apoptotic stimuli, suggesting that BIF acts as the trigger to activate Bax, and as suppression of BIF promoted HeLa cell colony formation in soft agar, it may have a role in the suppression of cancer progression. At least two isoforms of BIF are known to exist.
• Synonyms: Bif-1; CGI-61; dJ612B15.2; PPP1R70

Reference Data

• Protein Pathways: Endocytosis