UNG Rabbit Polyclonal Antibody

CAT#: TA306335

Rabbit Polyclonal UNG1 Antibody

Product Images

Western blot analysis of UNG1 in C2C12 cell lysate with UNG1 antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml.

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  Immunohistochemistry of UNG1 in human heart tissue with UNG1 antibody at 2 ug/ml.

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  Immunofluorescence of UNG1 in Human Heart cells with UNG1 antibody at 20 ug/mL.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 0.5 - 2 ug/mL, ICC: 2 ug/mL, IF: 20 ug/mL
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: UNG1 antibody was raised against a 14 amino acid peptide from near the carboxy terminus of human UNG1.
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1ug/ul
• Purification: Affinity chromatography purified via peptide column
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: uracil DNA glycosylase
• Database Link:
  NP_003353
  Entrez Gene 22256 MouseEntrez Gene 304577 RatEntrez Gene 7374 Human
  UniProt ID P13051
• Background: The human uracil-DNA glycosylase (UNG) gene encodes both mitochondrial (UNG1) and nuclear (UNG2) forms through differentially regulated promotes and alternative splicing. While UNG2 is the major enzyme in the base excision repair pathway that removes uracil residues from nuclear DNA that arise through either misincorporation during replication or cytosine deamination, inhibition of UNG1 by uracil glycosylase inhibitor did not lead to increased levels of spontaneous or induced mitochondrial DNA mutations. However, decreased levels of UNG activity and increased oxidative damage to mitochondrial DNA were seen in older mice, suggesting that mitochondrial DNA repair mechanisms may be involved in various neurodegenerative disorders in an age-dependent manner. This UNG1 antibody will not cross-react with UNG2.
• Synonyms: DGU; HIGM4; HIGM5; UDG; UNG1; UNG2; UNG15

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Base excision repair, Primary immunodeficiency