PAK2 Rabbit Polyclonal Antibody

CAT#: TA306346

Rabbit Polyclonal PAK2 Antibody

Product Images

Western blot analysis of PAK2 in Jurkat lysate with PAK2 antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml.

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  Figure from citation: Western Blot of PAK2 protein level by using anti-PAK2 antibody in cell lysates from the thymi of Pak2F/F (WT), Pak2F/+; Lck-Cre (HET) and Pak2F/F; Lck-Cre (KO) mice. View Citation

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  Immunohistochemistry of PAK2 in mouse spleen tissue with PAK2 antibody at 10 ug/ml.

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  Immunofluorescence of PAK2 in mouse spleen tissue with PAK2 antibody at 20 ug/mL.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 0.5 - 2 ug/mL, ICC: 10 ug/mL, IF: 20 ug/mL
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: PAK2 antibody was raised against a 14 amino acid peptide from near the amino terminus of human PAK2.
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1ug/ul
• Purification: Affinity chromatography purified via peptide column
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: p21 (RAC1) activated kinase 2
• Database Link:
  NP_002568
  Entrez Gene 29432 RatEntrez Gene 224105 MouseEntrez Gene 5062 Human
  UniProt ID Q13177
• Background: The p21-activated kinases (PAKs) are serine-threonine kinases that bind to the active forms of Cdc42 and Rac. They are divided into two groups, the first of which include PAK1, 2 and 3, and can be activated by Cdc42/Rac binding. Group 1 PAKs contain an autoinhibitory domain whose activity is regulated by Cdc42/Rac binding. The group 1 PAKs are known to be involved in cellular processes such as gene transcription, apoptosis, and cell morphology and motility. Much less is known about the second group, which includes PAK4, 5 and 6, and are not activated by Cdc42/Rac binding. Of the six PAK proteins, only PAK2 is ubiquitously expressed and cleaved by caspase-3. This cleavage removes the amino-terminal regulatory domain and generates a constitutively active kinase fragment. Recent experiments have shown that following cleavage, the active fragment is myristoylated and directed to the plasma membrane and membrane ruffles where it promotes cell death via increased signaling through the c-Jun N-terminal kinase pathway, but without compromising mitochondrial integrity.
• Synonyms: PAK65; PAKgamma

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Axon guidance, ErbB signaling pathway, Focal adhesion, MAPK signaling pathway, Regulation of actin cytoskeleton, Renal cell carcinoma, T cell receptor signaling pathway