ATM Rabbit Polyclonal Antibody

CAT#: TA306355

Rabbit Polyclonal ATM Antibody

Product Images

Western blot analysis of ATM in Daudi whole cell lysate with ATM antibody at (A) 1 and (B) 2 ug/ml.

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  Immunohistochemistry of ATM in human lymph node tissue with ATM antibody at 2.5 ug/ml.

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  Immunofluorescence of ATM in Human Lymph Node cells with ATM antibody at 10 ug/mL.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1 - 2 ug/mL, ICC: 2.5 ug/mL, IF: 10 ug/mL
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: ATM antibody was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human ATM.
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1ug/ul
• Purification: Affinity chromatography purified via peptide column
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: ATM serine/threonine kinase
• Database Link:
  NP_000042
  Entrez Gene 472 Human
  UniProt ID Q13315
• Background: DNA double strand breaks represent a major threat to an organism’s genome. Eukaryotic cells have developed mechanisms that sense the presence this damage and initiate suitable responses that can include DNA repair, cell cycle delay, and programmed cell death. The ATM (mutated in Ataxia-Telangiectasia) protein kinase is activated following the formation of DNA double strand breaks, phosphorylating p53 and another kinase CHK2. This initiates a signaling cascade leading to the phosphorylation and inhibition of Cdc25, ultimately preventing cell cycle progression. In some cell types, such as the hemapoietic system, this leads to apoptosis instead of cell cycle arrest. Multiple isoforms of ATM are known to exist.
• Synonyms: AT1; ATA; ATC; ATD; ATDC; ATE; TEL1; TELO1

Reference Data

• Protein Families: Druggable Genome, Protein Kinase, Transcription Factors
• Protein Pathways: Apoptosis, Cell cycle, p53 signaling pathway