TMP21 (TMED10) Rabbit Polyclonal Antibody

CAT#: TA306376

Rabbit Polyclonal TMP21 Antibody

Product Images

Western blot analysis of TMP21 in mouse brain tissue lysate with TMP21 antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml.

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  Immunohistochemistry of TMP21 in human brain tissue with TMP21 antibody at 5 ug/ml.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 0.5 - 2 ug/mL, ICC: 5 ug/mL
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: TMP21 antibody was raised against a 18 amino acid peptide from near the center of human TMP21.
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1ug/ul
• Purification: Affinity chromatography purified via peptide column
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: transmembrane p24 trafficking protein 10
• Database Link:
  NP_006818
  Entrez Gene 68581 MouseEntrez Gene 84599 RatEntrez Gene 10972 Human
  UniProt ID P49755
• Background: TMP21 is a ubiquitously expressed protein that is involved in vesicular targeting and protein transport. More recent experiments have shown that it is also a component in the presenilin complex and modulates the gamma-secretase but not theepsilonsecretase cleavage activity of the amyloid precursor protein. The presenilin complex is composed of the proteins APH1, nicastrin, and PEN2 in addition to presenilin-1. Together, these proteins cleave the amyloid precursor protein at what is known as the gamma and epsilon-sites and can lead to the accumulation of the Abeta cleavage product that is associated with Alzheimer’s disease. Co-immunoprecipitation experiments using antibodies against these proteins also yielded TMP21 indicating that TMP21 may play a role in the regulation of this complex. Suppression of TMP21 expression by siRNA in transfected cells caused increased gamma-secretase activity but not epsilon-secretase activity, and increased Abeta production, demonstrating that TMP21 can modulate gammasecretase activity.
• Synonyms: p23; P24(DELTA); p24d1; S31I125; S31III125; Tmp-21-I; TMP21

Reference Data

• Protein Families: Transmembrane