ORAI1 Rabbit Polyclonal Antibody

CAT#: TA306394

Rabbit Polyclonal ORAI1 Antibody

Product Images

Western blot analysis of ORAI1 in human ovary tissue lysate with ORAI1 antibody at 1 ug/ml in the (A) absence or (B) presence of blocking peptide.

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  Immunohistochemistry of ORAI1 in human spleen tissue with ORAI1 antibody at 10 ug/ml.

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  Immunofluorescence of ORAI1 in Human Spleen cells with ORAI1 antibody at 20 ug/mL.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1 ug/mL, ICC: 10 ug/mL, IF: 20 ug/mL
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: ORAI1 antibody was raised against a 18 amino acid peptide from near the amino terminus of human ORAI1.
• Formulation: PBS containing 0.02% sodium azide.
• Purification: Affinity chromatography purified via peptide column
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: ORAI calcium release-activated calcium modulator 1
• Database Link:
  NP_116179
  Entrez Gene 109305 MouseEntrez Gene 84876 Human
  UniProt ID Q96D31
• Background: Antigen stimulation of immune cells triggers Ca++ entry through Ca++ release-activated Ca++ (CRAC) channels. ORAI1 is a recently identified four-transmembrane spanning protein that is an essential component of CRAC. A missense mutation in this protein in humans is the cause of one form of hereditary severe combined immune deficiency (SCID) which results in ablated T-cell Ca++ entry. It has been suggested that ORAI1 functions as a highly selective Ca++ plasma membrane channel that is gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor. ORAI1 often migrates at a higher than expected molecular weight in SDS-PAGE. This antibody is predicted to have no cross-reactivity to ORAI2 or ORAI3.
• Synonyms: CRACM1; IMD9; ORAT1; TAM2; TMEM142A

Reference Data

• Protein Families: Transmembrane