PD L1 (CD274) Rabbit Polyclonal Antibody

CAT#: TA306401

Rabbit Polyclonal PD-L1 Antibody

Product Images

Western blot analysis of PD-L1 in Raji cell lysate with PD-L1 antibody at (A) 0.5 and (B) 1 ug/mL.

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  Immunohistochemistry of PD-L1 in human tonsil tissue with PD-L1 antibody at 5 ug/ml.

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  Immunohistochemistry of PD-L1 in rat heart tissue with PD-L1 Antibody at 5 ug/mL.

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  Immunohistochemistry of PD-L1 in human heart tissue with PD-L1 antibody at 2.5 ug/mL.

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  Immunofluorescence of PD-L1 in Human Heart cells with PD-L1 antibody at 20 ug/mL.

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  Immunofluorescence of PD-L1 in rat heart tissue with PD-L1 Antibody at 20 ug/mL.

• Flow cytometry analysis of A-20 cells using PD-L1 antibody at 0.5 ug/ml. Green: Isotype control. Red : PD-L1 antibody.

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Specifications

Product Data

• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB: 0.5 - 1 ug/mL, ICC: 2.5 ug/mL, IF: 20 ug/mL
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: PD-L1 antibody was raised against a 17 amino acid synthetic peptide from near the center of human PD-L1. The immunogen is located within amino acids 60 - 110 of PD-L1.
• Formulation: PBS containing 0.02% sodium azide.
• Purification: Affinity chromatography purified via peptide column
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: CD274 molecule
• Database Link:
  NP_054862
  Entrez Gene 60533 MouseEntrez Gene 499342 RatEntrez Gene 29126 Human
  UniProt ID Q9NZQ7
• Background: Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antigen-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. PDL-1 is a B7-related protein that inhibits cell-mediated immune responses by reducing the secretion of IL-2 and IL-10 from memory T cells. This suggests that PDL-1 may be useful in reducing allogenic CD4+ memory T-cell responses to endothelial cells, thereby reducing the likelihood of host immune responses to allografts. At least two isoforms of PDL-1 are known to exist; this antibody is specific to the larger isoform. PDL-1 antibody has no cross-reactivity to PDL-2.
• Synonyms: B7-H; B7H1; PD-L1; PDCD1L1; PDCD1LG1; PDL1

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs)