Stromal interaction molecule 1 (STIM1) Rabbit Polyclonal Antibody

CAT#: TA306421

Rabbit Polyclonal STIM1 Antibody

Product Images

Western blot analysis of STIM1 in mouse thymus tissue lysate with STIM1 antibody at (A) 1 and (B) 2ug/ml.

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  Immunohistochemistry of STIM1 in mouse spleen tissue with STIM1 antibody at 5ug/ml.

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  Immunohistochemistry of STIM1 in human spleen tissue with STIM1 antibody at 2.5ug/ml.

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  Figure from citation: Immunohistochemistry of STIM1 protein level by using anti-STIM1 antibody in muscle type II fibers in Stormorken syndrome patients. Dilution: 1:200 View Citation

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  Immunofluorescence of STIM1 in mouse spleen tissue with STIM1 antibody at 20ug/ml.

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  Immunofluorescence of STIM1 in Hek293 cells with STIM1 antibody at 20ug/ml.

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Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1 - 2 ug/mL, ICC: 2.5 ug/mL
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: STIM1 antibody was raised against a 24 amino acid synthetic peptide from near the carboxy terminus of human STIM1. The immunogen is located within the last 50 amino acids of STIM1.
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Affinity chromatography purified via peptide column
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: stromal interaction molecule 1
• Database Link:
  NP_003147
  Entrez Gene 20866 MouseEntrez Gene 361618 RatEntrez Gene 6786 Human
  UniProt ID Q13586
• Background: In T lymphocytes, the sole pathway for Ca++ entry following antigen-receptor binding is through store-operated Ca++-release-activated Ca++ (CRAC) channels. These channels are made up of the pore-forming subunit ORAI1 and the stromal interaction molecule 1 (STIM1), a protein that functions as a Ca++ sensor and activates the CRAC channels, migrating to the plasma membrane from endoplasmic reticulum (ER)-like sites which act as the Ca++ store. A related molecule, STIM2, acts to inhibit the STIM1-mediated store-operated Ca++ entry, and can form complexes with STIM1, suggesting they may play a coordinated role in controlling Ca++ entry. At least two isoforms of STIM1 are known to exist; this antibody will detect only the larger form. This STIM1 antibody is predicted to have no cross-reactivity to STIM2.
• Synonyms: D11S4896E; GOK; IMD10; STRMK; TAM; TAM1

Reference Data

• Protein Families: Transmembrane